Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Histamine release from the basophils of control and asthmatic subjects and a comparison of gene expression between "releaser" and "nonreleaser" basophils.

doi: 10.4049/jimmunol.178.7.4584

Figure Lengend Snippet: FIGURE 3. Histamine release from the five releasers and three nonre- leasers basophils on the same day as RNA purification. Percoll-enriched releaser and nonreleaser basophils were incubated for 30 min in various concentrations of anti-IgE or A23187 (5 ng/ml), and histamine levels were measured in the incubation supernatants using ELISA. Results, corrected for spontaneous release, show the range of duplicate assays. Anti-IgE in- duced no histamine release from NR116 and NR130.

Article Snippet: The IL-3 ELISA kit was from R&D Systems, and the human histamine kits were from Beckman Coulter.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Histamine release from the basophils of control and asthmatic subjects and a comparison of gene expression between "releaser" and "nonreleaser" basophils.

doi: 10.4049/jimmunol.178.7.4584

Figure Lengend Snippet: FIGURE 5. Time course and dose-response studies of anti-IgE-induced basophil chemokine release. In A–C, releaser (solid lines) or nonreleaser (dashed lines) basophils were incubated for 1, 2, or 4 h with 0.1 g/ml anti-IgE. In D–F, releaser (solid bars) or nonreleaser (striped bars) basophils were incubated with 0.01, 0.1, 1, and 5 g/ml anti-IgE or 500 ng/ml A23187 for 4 h at 37°C. Levels of IL-8 (A), MIP-1 (B), and MIP-1 (C) in the culture supernatants were analyzed by ELISA.

Article Snippet: The IL-3 ELISA kit was from R&D Systems, and the human histamine kits were from Beckman Coulter.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay